Osteogenic potential of adult stem cells from human maxillary sinus membrane by Simvastatin in vitro: preliminary report
윤경인, 김동준, 박재억,
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윤경인 ( Yun Kyoung-In ) - Catholic University College of Medicine St. Paul’s Hospital Department of Oral and Maxillofacial Surgery
김동준 ( Kim Dong-Joon ) - Catholic University School of Medicine Department of Oral and Maxillofacial Surgery
박재억 ( Park Je-Uk ) - Catholic University School of Medicine Department of Oral and Maxillofacial Surgery
KMID : 0355620130390040150
Abstract
Objectives: The objective of this study is to determine the adequate concentration and to evaluate the osteogenic potential of simvastatin in human maxillary sinus membrane-derived stem cells (hSMSC).
Materials and Methods: Mesenchymal stem cells derived from the human maxillary sinus membrane were treated with various concentrations of simvastatin. The adequate concentration of simvastatin for osteogenic induction was determined using bone morphogenetic protein (BMP-2). The efficacy of osteogenic differentiation of simavastatin was verified using osteocalcin mRNA, and the mineralization efficacy of hSMSCs and simvastatin treatment was compared with alkaline phosphatase and von Kossa staining.
Results: Expression of BMP-2 mRNA and protein was observed after three days and was dependent on the concentration of simvastatin. Expression of osteocalcin mRNA was observed after three days in the 1.0 ?M simvastatin-treated group. Mineralization was observed after three days in the simvastatin-treated group.
Conclusion: These results suggest that simvastatin induces the osteogenic potential of mesenchymal stem cells derived from the human maxillary sinus membrane mucosa.
키워드
Adult stem cells; Maxillary sinus; Simvastatin
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